Figure 3.

Effects of age on human PSC yield, viability, and frequency. Samples were derived from both genders and a wide range of ages (24–69 years). Characteristics of cell yield, viability, and frequency were assessed, stratified by age. Parameters of yield, viability, and frequency were as follows. (A): Total SVF cell yield, as represented by total SVF cells (10⁶) per 100 ml of tissue lipoaspirate. (B): Percentage of cell viability, as determined by percentage of DAPI− cells. (C): Percentage of hematopoietic cells, as determined by percentage of CD45+ cells among DAPI− cells. (D): Percentage of pericytes, as determined by percentage of CD146+, CD34− cells among DAPI− cells. (E): Percentage of adventitial cells, as determined by percentage of CD146−, CD34+ cells among DAPI− cells. (F): Percentage of human PSCs, as determined by percentage of pericytes + percentage of adventitial cells. Abbreviations: PSC, perivascular stem cell; SVF, stromal vascular fraction.