Figure 2.

Schematic analysis of the procedure recently used to generate beta cells from human fetal pancreases [1]. Immature human fetal pancreases were transduced with lentiviral vectors that expressed SV40T under the control of the insulin promoter. Transduced pancreases were next transplanted under the kidney capsule of immunoincompetent SCID mice where insulinomas developed, from which human beta-cell lines were derived. Abbreviations: LTR, long terminal repeat; Ins, insulin; SV40T, large-T antigen of simian virus 40.