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. 2013 Jan 22;2(2):118–128. doi: 10.5966/sctm.2012-0092

Figure 4.

Figure 4.

Expression of senescence-associated genes in iPSC lines. (A): Quantitative real-time reverse transcription-polymerase chain reaction (PCR) for p21, p53, and Bax genes indicated no difference (p > .05) in the expression of senescence-associated genes in three induced pluripotent stem clones of each cell line. The mRNA expression level is shown as the average of three clones of each cell line (passage 9). C1, C3, and C64 were derived from the older donor; C15, C16, and C42 were derived from the younger donor). The reactions were normalized against internal control (GAPDH). Fibroblasts isolated from the vaginal wall of a 34-year-old subject were used a calibrator for real-time PCR. HT1080 cells were used a positive control. (Bi): Representative Western blots of p53, p21, and Bax from two clones (passage 9) from each cell line were analyzed. GAPDH was used a loading control. (Bii): The average expression levels of three clones (passage 9) derived from an older and a younger donor indicate no significant difference between groups. Abbreviations, GAPDH, glyceraldehyde-3-phosphate dehydrogenase; iPSC, induced pluripotent stem cell.