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. 2013 Feb 19;2(3):161–166. doi: 10.5966/sctm.2012-0073

Figure 1.

Figure 1.

Generation of NTG and TG (R120G αB-crystallin [CryAB]) mouse iPSCs. (A): Morphologies of mouse iPSCs in SNL feeder and feeder-independent cultures. Scale bars = 400 μm (left), 200 μm (right). (B): Genotyping of induced pluripotent stem colonies from NTG-11, NTG-64, TG-11, and TG-18 along with NTG-TTFs and TG-TTFs used as controls. Arrow indicates the amplified polymerase chain reaction (PCR) product in transgenic samples. (C): Immunofluorescence staining of pluripotent markers OCT4 (green) and NANOG (red) in representative iPSC and embryonic stem (ES) cell (E14, mouse embryonic stem cells) clones. An inverted fluorescent microscope was used to image the samples, and pictures were equally treated using autocontrast enhancement. Scale bar = 100 μm. (D): Reverse transcription-PCR analysis of embryonic stem cell marker genes in mouse iPSCs, E14, and TTFs. We used primers that amplified endogenous but not transgenic transcripts. (A full list of abbreviations is given in the supplemental online data.) Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; iPSC, induced pluripotent stem cell; NTG, nontransgenic; TG, transgenic; TTF, tail tip fibroblast.