Figure 5.

The biphasic effect of MSC dose on expression of rat CNP in cocultures. (A): The CNP gene expression assessed using quantitative reverse transcription-polymerase chain reaction was directly dependent on MSC dose on day 5 but was inhibited by higher MSC doses on day 7. Values are expressed relative to the highest level on each day. (B): MSCs promoted CNP protein expression; however, lower MSC doses promoted more advanced oligodendrocyte differentiation on day 12 than did higher doses. Magnification, ×200. Abbreviations: CNP, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; MSC, mesenchymal stromal cell; Rel., relative.