Figure 6.

MSC-derived FGF2 mediates the MSC-driven nestin increase in cocultures; quantitative reverse transcription-polymerase chain reaction. (A): Heparinase I pretreatment of extracellular matrix-coated wells reduced basal levels of Nes in neural cultures. (B): Neural cells and MSCs, 200 cells per well, were cocultured in the presence or absence of isotype-matching anti-FGF2 antibodies, either bFM1 (neutralizing) or bFM2 (non-neutralizing). (C): Neural cells were cultured in the presence or absence of 40% MSC-CM, either intact, FGF2-immunodepleted, or control-treated. Abbreviations: CM, mesenchymal stromal cell-conditioned medium; FGF2, fibroblast growth factor 2; IP, immunoprecipitation; MSC, mesenchymal stromal cell; Nes, nestin; Rel., relative.