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. 2013 Mar 22;2(4):265–273. doi: 10.5966/sctm.2012-0154

Figure 2.

Figure 2.

High molecular weight proteins of >240 kDa are glycoprotein ligands of rBC2LCN in human iPS cells and ES cells. One microgram of hydrophilic fractions of somatic cells, iPS cells, and ES cells was run on 5%–20% acrylamide gel under reducing conditions, electroblotted onto polyvinylidene difluoride membrane, and stained with 1 μg/ml of HRP-conjugated rBC2LCN (top panel). Silver staining was also performed (bottom panel). Abbreviations: ES, embryonic stem; HRP, horseradish peroxidase; iPS, induced pluripotent stem; MEF, mouse embryonic fibroblasts.