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. 2013 Mar 22;2(4):265–273. doi: 10.5966/sctm.2012-0154

Figure 5.

Figure 5.

Glycan ligands on podocalyxin recognized by rBC2LCN are O-glycans. The podocalyxin immunoprecipitated from induced pluripotent stem cells (iPS201B7 [P45], 123; lanes 1 and 2) and embryonic stem cells (KhES-1 [P26], 156; lanes 3 and 4) with or without PNGase F treatment was run on 5%–20% acrylamide gel under reducing condition and transferred onto polyvinylidene difluoride membranes. The membranes were treated with phosphate-buffered saline (left panel) or 0.05 M NaOH (right panel) and blotted with horseradish peroxidase-conjugated rBC2LCN. Lane 5 shows Fucα1–2Galβ1–3GlcNAcβ1–3Galβ1–4Glc-BSA (rBC2LCN ligand, 0.5 μg). Abbreviation: PNGase F, Peptide:N-glycosidase F.