Figure 2.

Human pluripotent stem cell (hPSC) growth in feeder-free monolayer culture and in colonies on mEFs. (A): Growth kinetics of hPSCs in monolayer culture. Splitting of cells, performed at a 1:20 ratio after 72 hours of culture, is indicated. R² (coefficient of determination) indicates the plausibility of exponential growth. Error bars indicate SEM (n = 5). (B): Growth kinetics of hPSCs in mEF-dependent colony culture. Error bars indicate SEM (n = 5). (C): Colony-forming efficiency of hPSCs during the initial 15 passages in monolayer culture. Error bars indicate SEM (n = 6). (D): Teratoma-forming efficiency of human embryonic stem cells (hESCs) in monolayer culture or in colonies on mEFs. Data were generated from CCTL12 and CCTL14 cells. The numbers in the columns show the number of mice that developed teratomas compared with the total number of mice that received intramuscular injections of hESCs. Error bars indicate SEM. Abbreviations: hiPSC, human induced pluripotent stem cell; mEF, murine embryonic fibroblast.