Figure 3.

Expanded nTregs control GvHD in a xenogeneic mouse model. Irradiated NSG mice were infused with PBMC either alone or in combination with expanded nTregs (S3) or expanded control CD25^Dep cells at 1:1 ratio. (A) The graph illustrates the measurements of the weight of the NSG mice. Data show mean ± SD of 12 animals for each group (*P=0.0001). (B) Images show that control mice developed signs of GvHD, such as hair loss and orbital tightening. (C) Spleen enlargements were observed in mice infused with control CD25^Dep cells. (D) Representative H&E and immunohistochemistry staining for CD8⁺ cells in tissue sections obtained from skin, nose, ear, and spleen of treated mice. Control mice showed evidence of chronic dermatitis, with moderate diffuse epithelial hyperplasia, hyperkeratosis and marked multi-focal coalescing subcutaneous and dermal mononuclear inflammatory cell infiltrates. Arrows indicate infiltrating CD8⁺ cells. (E) Kaplan-Meier survival curve comparing NSG mice receiving human PBMC alone or in combination with expanded nTregs or expanded control CD25^Dep cells (12 animals for each group) (*P=0.0003).