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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Diabetologia. 2012 Aug 17;55(11):2989–2998. doi: 10.1007/s00125-012-2678-y

Fig. 3. NEP cleavage of hIAPP.

Fig. 3

hIAPP (20 μmol/l) was incubated with 400 nmol/l of purified NEP at 37°C for 60 min in the absence (a) or presence (b) of the NEP inhibitor-phosphoramidon (PA) or 400 nmol/l of catalytically compromised NEP (NEPx) (c). Reactions were stopped with 0.5% TFA and analyzed by HPLC as described in Materials and Methods. hIAPP was cleaved by NEP (a) but not by PA-treated NEP (b) or NEPx (c). The large peaks at retention time at 20–30 min in panel b are the phosphoramidon peaks.