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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Diabetologia. 2012 Aug 17;55(11):2989–2998. doi: 10.1007/s00125-012-2678-y

Fig. 6. Effect of recombinant NEP or NEPx on synthetic hIAPP-induced cytotoxicity.

Fig. 6

Insulinoma INS 832/13 cells were treated with vehicle (0.8% DMSO in media), 20μmol/l synthetic rIAPP or 20 μmol/l synthetic hIAPP along with 4, 40 nmol/l or 400 nmol/l recombinant NEP or NEPx. 400 nmol/l transferrin (TF) was used as control.

a. After treatment for 48 h cell viability was determined using the MTT assay.

## p<0.01 compared to the vehicle control.

** P<0.01 compared to hIAPP alone.

(n = 3 to 9).

b. Cell apoptosis was evaluated 24 h after treatment with IAPP by following the cleavage and activation of caspase-3 by Western blot analysis. Exogenously added NEP, but not NEPx, reduced cleaved caspase-3 levels induced by synthetic hIAPP.