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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Transgenic Res. 2007 Oct 30;17(2):265–279. doi: 10.1007/s11248-007-9152-5

Fig. 4.

Fig. 4

Immunoblot of EGFP expression at 24 hpf and 7 dpf. Total protein extracts from AB and five transgenic lines were isolated at 24 hpf and 7 dpf, electrophoresed through 4–12% SDS-PAGE, and transferred to PVDF membrane. Duplicate membranes were incubated with either anti-GFP or anti-actin antibodies, which served as a loading control. The immunoblots revealed a decrease in EGFP expression from 24hfp to 7 dpf in the Tg(h2afv:EGFP)nt13 line and increased expression in the Tg(eef1g:EGFP)nt15 and the Tg(tbnl:EGFP)nt16 lines. The Tg(tbnl:EGFP)nt16 line also revealed an EGFP protein that was slightly larger than the EGFP in the other transgenic lines. This increased molecular weight is due to an AUG codon that is in frame and upstream of the EGFP open reading frame, which yields a fusion protein