Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Diagram displaying the principle of digital polymerase chain reaction (PCR). A bulk PCR reaction is partitioned into small volume aliquots that may or may not lack template. After thermal cycling, aliquots that contained at least 1 template molecule will show a positive signal. The ratio of positive to negative reactions is then analyzed using Poisson statistics to predict the absolute copy number in the original bulk sample.
