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Distribution of fluorescence events after ddPCR analysis of complementary DNA from heterozygous Huntington's Disease patient-derived cells. Frequency is the number of droplets. Amplitude is the signal in a given droplet generated by the release of fluorophore from hydrolyzed probe and is proportional to the number of amplicons within a droplet. Events due to both mutant and wild-type huntingtin (HTT) protein mRNA are shown.
