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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Exp Hematol. 2012 Oct 17;41(2):180–188.e4. doi: 10.1016/j.exphem.2012.10.007

Figure 2.

Figure 2

The consequences of thymineless death following FdUMP[10] treatment are effectively rescued with exogenous thymidine only when administered during the first replicative cycle (<18 hours). (A) Depiction of the experimental scheme used for Thy rescue experiments and the relative timing of induction of a thymineless state, Top1CC formation, and apoptotic cell death. (B) Cell viability assays showing the time dependence of Thy rescue following FdUMP[10] treatment (10−8 mol/L). (C) Induction of apoptotic cell death and time dependence of rescue by exogenous Thy (10−8 mol/L). Data are a summary of two to six experiments performed in triplicate with significant differences between adjacent time points indicated (time points being farther apart was also significant). (D) In vivo complex of enzyme (ICE) bioassays showing the induction of Top1CC formation following FdUMP[10] treatment (10−8 mol/L) and the time dependence of Thy rescue.