Stimulation of calcineurin and NFAT activation in aging cardiomyocyts. Calcineurin, NFAT and p-NFAT protein expression were increased in aging and aging exposure to SHS by western blot analysis. Quantification of densitometry analysis of protein levels. *p<0.05, **p<0.01, significantly difference compared with the male young control (MYC). #p<0.05, ##p<0.01, significantly difference compared with the male old control (MOC).