Table 2.
Total GPx and GPx4-specific activity in Coho liver and olfactory rosettes using different hydroperoxide substrates*
| Tissue | Substrate | Mitochondrial GPx or GPx4 (μmol/min per mg protein) | Cytosolic GPx or GPx4 (μmol/min per mg protein) |
|---|---|---|---|
| Liver1 | CuOOH | 28.4 ± 3.90 | 53.2 ± 3.51 |
| PCOOH | 10.6 ± 0.53 | 12.3 ± 1.01 | |
| Olfactory rosettes2 | CuOOH | 62.6 ± 6.38 | 96 ± 7.72 |
| PCOOH | 7.8 ± 1.70 | 9.1 ± 1.04 |
Activities were measured as described under methods, section 2.4. The GSH concentrations in all assays were fixed as 3 mM.
Enzymatic values in the liver tissue reflect the mean ± S.E. of 3 individual fish.
Enzymatic values in the olfactory rosettes reflect the mean ± S.E. of n=3 pools.