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. 2013 May 13;6:196. doi: 10.1186/1756-0500-6-196

Figure 2.

Figure 2

Pairwise variation (V) analysis of the candidate reference genes as predicted by geNorm. The Pairwise variation (Vn/Vn+1) was analyzed using the normalization factors NFn and NFn+1 to determine the optimal number of reference genes required for effective normalization of qPCR data.