Table 1.
Primer sequences and amplicon characteristics of tested genes
| Gene symbol | Forward primer sequence (5’- 3’) | Reverse primer sequence (5’- 3’) | Amplicon length (bp) | Primer location* | Efficiency (%) |
|---|---|---|---|---|---|
|
GmCYP2 |
CGGGACCAGTGTGCTTCTTCA |
CCCCTCCACTACAAAGGCTCG |
154 |
S |
98,3 |
|
GmELF1A |
GACCTTCTTCGTTTCTCGCA |
CGAACCTCTCAATCACACGC |
195 |
D |
102,4 |
|
GmTUA5 |
AGGTCGGAAACTCCTGCTGG |
AAGGTGTTGAAGGCGTCGTG |
159 |
S |
101,7 |
|
GmELF1B |
GTTGAAAAGCCAGGGGACA |
TCTTACCCCTTGAGCGTGG |
118 |
D |
92,5 |
|
GmACT11 |
CGGTGGTTCTATCTTGGCATC |
GTCTTTCGCTTCAATAACCCTA |
142 |
D |
104,1 |
|
GmUBC2 |
TCCCCTCACACCCTTCCTC |
CCATCCCAAGGGGTGTCAT |
155 |
D |
107,6 |
|
GmTUB |
CCTCGTTCGAATTCGCTTTTTG |
CAACTGTCTTGTCGCTTGGCAT |
161 |
S |
96,4 |
|
GmG6PD |
ACTCCTTGATACCGTTGTCCAT |
GTTTGTTATCCGCCTACAGCCT |
126 |
D |
110,7 |
|
GmUBC4 |
GAGCGAGCAGTTTCAGAC |
CATAGGAGGGACGATACG |
168 |
D |
98 |
| GmRB7 | TTGTAGGTGTCTCCGTCGC | AATGCTCTTGGCGGTGATG | 179 | S | 87,3 |
*Single exon (S) and different exons (D).