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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2013 Apr 24;190(11):5526–5533. doi: 10.4049/jimmunol.1201885

Figure 2. Serum immunoglobulin levels are decreased in immunized Lgals1−/− mice.

Figure 2

WT and Lgals1−/− mice were immunized with NP-Ficoll (T-independent antigen), 7 mice for each group (A) and with NP-KLH in alum (T-dependent antigen), 5 mice for each group (B, C) and Ig secretion in serum was quantified by ELISA. NP-specific IgM and IgG3 levels were determined at D2, D5, D8 and D15 for the T-independent responses (A). NP-specific IgM (B), low (NP23) and high (NP3) NP-specific IgG1 serum titers (C) were analyzed at D2, D5, D15, D18, and D21 for T-dependent responses. (D) Affinity maturation of T-dependent immune responses at days 18 and 21 was measured by the NP3 (high) / NP23 (low) affinity IgG1 ratio. Open and black squares correspond to WT and Lgals1−/− mice, respectively.

* and ** indicate significance versus WT mice with p<0.05 and <0.005 respectively.