Table I.
Antibodies for flow cytometry
| Reactivity | Source | Clone | Conjugate | Supplier |
|---|---|---|---|---|
| Primary mAb | ||||
| Mouse B220 | Rat | RA3-6B2 | FITC | eBioscience |
| Mouse B220 | Rat | RA3-6B2 | Pacific blue | eBioscience |
| Mouse CD21 | Rat | 7G6 | APC | BD Biosciences |
| Mouse CD23 | Rat | B3B4 | PE-Cy7 | eBioscience |
| Mouse CD93 | Rat | AA4.1 | PE | eBioscience |
| Mouse CD138 | Rat | 281-2 | biotin | BD Pharmingen |
| Mouse Galectin 1 | Rat | 201002 | Alexa 647*, Alexa 546* | R&D |
| Mouse Gl7 | Gl7 | biotin | eBioscience | |
| Mouse IgM | Rat | II/41 | PE-Cy5.5 | eBioscience |
| Streptavidin | PE-Cy5 | eBioscience | ||
| Streptavidin | APC-Cy7 | eBioscience |
*
Conjugated in our laboratory using the antibody labeling kit from Molecular Probes.