Table 2.
Details of the SNVs studied for DAZ, GOLY, BPY2 and TTTY4 genes
| Target | SNV | Oligos used | Accession no. | Product size | Enzyme for digestion | Restriction site | Fragments | Alleles | Present in copies | DU145 | LNCaP |
|---|---|---|---|---|---|---|---|---|---|---|---|
| |
DAZ-SNV_I |
SA 770 CACAGGCACTCAGTAACTATCTC |
G73167 |
709 |
Fsp1 |
TGC/GCA |
709 |
A |
1,2,3 4 |
A&B |
A&B |
| 398 + 311 |
B |
||||||||||
| SA771CAGTGTTTCACCCACCACTTCTGGGT | |||||||||||
| |
DAZ-SNV_II |
SA 446 GACATCCACGTCATTAACAAACG |
G73166 |
182 |
Mbo1 |
/GATC |
182 |
A |
1 2,3,4 |
A&B |
A&B |
| 122 + 60 |
B |
||||||||||
| SA 447 GGAAGCTGCTTTGGTAGATAC | |||||||||||
| |
DAZ-SNV_III(sY586) |
SA 772 GTGTGGCACATATGCCTATAAA |
G63907 |
301 |
Taq1 |
T/CGA |
301 |
A |
2 1,3,4 |
A&B |
A&B |
| 184 + 117 |
B |
||||||||||
| SA 773 TTGGTACATCCAGATGCAGAT | |||||||||||
|
DAZ gene |
DAZ-SNV_IV |
SA 774 CTTCCTCATCTTTCTTGACTT |
G73168 |
630 |
AluI |
AG/CT |
630 |
A |
2 1,3,4 |
A&B |
A&B |
| 398 + 262 |
B |
||||||||||
| SA 775 TTATTTATTCCTCAAAAAGGTG | |||||||||||
| |
DAZ-SNV_V (sY587) |
SA 444 TGGTTAATAAAGGGAAGGTGTTTT |
G63908 |
244 |
DraI |
TTT/AAA |
195 + 49 |
A |
3,4 1,2 |
A&B |
A&B |
| 122 + 73 + 49 |
B |
||||||||||
| SA 445 TCTCCAGGACAGGAAAATCC | |||||||||||
| |
DAZ-SNV_VI |
SA 776 GGGCCTAGTCTCTAGATCATT |
G73169 |
431 |
AflIII |
A/CRYGT |
431 |
A |
1,2,3 4 |
A&B |
A |
| 248 + 183 |
B |
||||||||||
| SA 777 GCTAGAACCAAATATTCTGGAT | |||||||||||
| |
DAZ-SNV_VII (sY581) |
SA 442 CACTGCCCTAATCCTAGCACA |
G63906 |
252 |
Sau3AI |
/GATC |
189 + 63 |
A |
1,4 2,3 |
A&B |
A&B |
| 130 + 63 + 59 |
B |
||||||||||
| SA 443 TCTTCTGGACATCCACGTCA | |||||||||||
|
GOLY1 |
GOLY-SNV_1 |
SA768 TTGGCCTGTTGCTTCTAGGGTT |
BV012733 |
531 |
HhaI |
GCG/C |
531 |
A |
1 Copy |
A&B |
A&B |
| SA769 ACAGGGAGGGTGCTGTCACA |
289 + 282 |
B |
1 Copy |
||||||||
|
BPY2 |
BPY2 |
SA766 AAGCCCATTGCTGAGATACTG |
BV012732 |
470 |
EcoRV |
GAT/ATC |
470 |
A |
2 Copy |
A&B |
A |
| SA767 TTGTGATTCTGACCCAACGA |
289 + 181 |
B |
1 Copy |
||||||||
| TTTY4 | TTTY4 | SA764 TGCAGACAGCACTGTGGCTT |
BV012731 | 541 | HaeIII | GG/CC | 541 |
A |
1 Copy |
A&B | A&B |
| 323 + 218 | B | 2 Copy | |||||||||
| SA765 GTATATGGCATAATTTCACCTG |