Table 3.
List of primers used for PCR amplification of DYZ1 array (A) and SRY gene (B)
|
(A) | |||||
|---|---|---|---|---|---|
| Serial No. | Primer ID | Primer Sequence | Length (bp) | Location (DYZ1 array) | Orientation |
|
1 |
SAS 1 |
CCATTCGAGACCGTAGCAAT |
20 |
35-16 (5’ upstream to HaeIII site) |
5’-3’ |
|
2 |
SAS 2 |
TTTCCTTTCGCTTGCATTCCAT |
22 |
63-84 |
5’-3’ |
|
3 |
SAS 3 |
ATTTGATGCCATCCCATGAC |
20 |
763-782 |
5’-3’ |
|
4 |
SAS 4 |
TTTTGAGTCCGTTCCATAACAC |
22 |
1380-1401 |
5’-3’ |
|
5 |
SAS 5 |
TCCTTTGCCTTCCATTCG |
18 |
1668-1685 |
5’-3’ |
|
6 |
SAS 6 |
TGCAGTCTTTTCCCTTCGAG |
20 |
2564-2583 |
5’-3’ |
|
7 |
SAS 7 |
ATTGGATGGGATTGGAATGA |
20 |
861-880 |
3’-5’ |
|
8 |
SAS 8 |
TGGATGGACTGCAATAGAAAG |
22 |
1600-1621 |
3’-5’ |
|
9 |
SAS 9 |
TCGAATGGAAGGCAAAGG |
18 |
1669-1686 |
3’-5’ |
|
10 |
SAS 10 |
CGACTGGTACGGACTCCAAT |
20 |
2637-2656 |
3’-5’ |
|
11 |
SAS 11 |
GACTGGAAAGGCTGGGTGTCGA |
22 |
3419-3440 |
3’-5’ |
|
12 |
SAS 12 |
TGGACAGCCTGGAATAAAGTG |
21 |
3586-3606 |
3’-5’ |
|
(B) | |||||
|
1 |
SA 531 |
GAATCTGGTAGAAGTGAGTTTTGGA |
25 |
61-85 |
5’-3’ |
| 2 | SA 532 | GCCTTTATTAGCCAGAGAAAAGAAA | 25 | 860-884 | 3’-5’ |