Table 4.
List of DYZ1 primer combinations used for end point PCR, their corresponding amplicon sizes and reaction conditions
| Serial No. | DYZ1, Primer Combinations | Amplicon Size (bp) | PCR conditions (Annealing and Extension) |
|---|---|---|---|
|
1 |
SAS (1&7) |
915 |
64°C-1.0’, 72°C −1.0’ |
|
2 |
SAS (1&8) |
1656 |
65°C −1.0’, 72°C −2.0’ |
|
3 |
SAS (1&9) |
1721 |
61°C −1.0’, 72°C −2.0’ |
|
4 |
SAS (2&7) |
818 |
61°C −1.0’, 72°C −1.0’ |
|
5 |
SAS (2&8) |
1559 |
60-°C 1.0’, 72°C −1.5’ |
|
6 |
SAS (3&7) |
118 |
55-°C 1.0’, 72°C −1.0’ |
|
7 |
SAS (3&8) |
859 |
65°C −1.0’, 72°C −1.0’ |
|
8 |
SAS (3&9) |
924 |
61°C −1.0’, 72°C −1.0’ |
|
9 |
SAS (3&10) |
1894 |
64-°C 1.0’, 72°C −2.0’ |
|
10 |
SAS (4&8) |
242 |
61°C −1.0’, 72°C −1.0’ |
|
11 |
SAS (4&9) |
307 |
55°C −1.0’, 72°C −1.0’ |
|
12 |
SAS (4&10) |
1277 |
61°C −1.0’, 72°C −1.5’ |
|
13 |
SAS (4&11) |
2061 |
62°C −1.0’, 72°C −2.5’ |
|
14 |
SAS (4&12) |
2192 |
61°C −1.0’, 72°C −2.5’ |
|
15 |
SAS (5&10) |
989 |
61°C −1.0’, 72°C −1.0’ |
|
16 |
SAS (5&12) |
1904 |
61°C −1.0’, 72°C −2.0’ |
|
17 |
SAS (6&10) |
93 |
61°C −1.0’, 72°C −1.0’ |
|
18 |
SAS (6&11) |
877 |
61°C −1.0’, 72°C −1.0’ |
| 19 | SAS (6&12) | 1008 | 64°C −1.0’, 72°C −1.0’ |