Table 6.

Restriction mapping of 3.56 Kb DYZ1 sequence from DU145, LNCaP and the reference sequence AC068123.5 by virtual digest using Restriction Mapper Version 3 software

Serial No.	Restriction enzyme	Sequence	Sight Length	Overhung	Frequency of cut sites
					AC068123.5	DU145	LNCaP
1	BsaBI	GATNNNNATC	6	blunt	1	0	0
2	Cac8I	GCNNGC	4	blunt	1	1	1
3	NlaIV	GGNNCC	4	blunt	1	0	0
4	BamHI	GGATCC	6	5’	1	0	0
5	BseYI	CCCAGC	6	5’	1	1	1
6	Bsp1407I	TGTACA	6	5’	1	1	1
7	BspHI	TCATGA	6	5’	1	1	1
8	ClaI	ATCGAT	6	5’	1	1	1
9	DdeI	CTNAG	4	5’	1	1	1
10	EcoRI	GAATTC	6	5’	1	1	1
11	EcoRII	CCWGG	5	5’	1	0	0
12	MaeII	ACGT	4	5’	1	1	0
13	MaeIII	GTNAC	4	5’	1	1	0
14	VspI	ATTAAT	6	5’	1	1	1
15	XhoII	RGATCY	6	5’	1	0	0
16	BseMII	CTCAG	5	3’	1	1	1
17	BsgI	GTGCAG	6	3’	1	1	1
18	BtsI	GCAGTG	6	3’	1	1	1
19	Eco57I	CTGAAG	6	3’	0	1	1
20	Eco57MI	CTGRAG	6	3’	1	2	2
21	GsuI	CTGGAG	6	3’	1	1	1
22	Hpy188I	TCNGA	4	3’	1	1	1
23	MnlI	CCTC	4	3’	1	1	1
24	PflMI	CCANNNNNTGG	6	3’	1	1	1
25	SduI	GDGCHC	6	3’	1	1	1
26	ScrFI	CCNGG	4	5’	2	1	1
27	ArsI	GACNNNNNNTTYG	7	3’	2	2	2
28	BdaI	TGANNNNNNTCA	6	3’	2	2	2
29	CspCI	CAANNNNNGTGG	7	3’	2	0	0
30	TspRI	CASTG	5	3’	2	2	2
31	DpnI	GATC	4	blunt	3	2	2
32	ApoI	RAATTY	6	5’	3	3	3
33	AsuII	TTCGAA	6	5’	3	3	3
34	Eco31I	GGTCTC	6	5’	3	2	2
35	MaeI	CTAG	4	5’	3	3	4
36	MboI	GATC	4	5’	3	2	2
37	SfaNI	GCATC	5	5’	3	4	4
38	TatI	WGTACW	6	5’	3	3	3
39	SetI	ASST	4	3’	3	3	1
40	CviJI	RGCY	4	blunt	4	4	4
41	MslI	CAYNNNNRTG	6	blunt	4	4	4
42	FokI	GGATG	5	5’	4	2	2
43	AlfI	GCANNNNNNTGC	6	3’	4	4	4
44	RsaI	GTAC	4	blunt	5	5	4
45	BccI	CCATC	5	5’	5	3	4
46	BsmAI	GTCTC	5	5’	5	5	4
47	MseI	TTAA	4	5’	5	6	6
48	BstXI	CCANNNNNNTGG	6	3’	5	5	4
49	MboII	GAAGA	5	3’	5	3	3
50	XmnI	GAANNNNTTC	6	blunt	6	6	6
51	TstI	CACNNNNNNTCC	6	3’	6	4	4
52	NlaIII	CATG	4	3’	7	7	7
53	BcgI	CGANNNNNNTGC	6	3’	16	14	14
54	BsrDI	GCAATG	6	3’	16	14	14
55	BsrI	ACTGG	5	3’	17	19	18
56	TspGWI	ACGGA	5	3’	20	23	17
57	AgsI	TTSAA	5	3’	22	20	23
58	BsmI	GAATGC	6	3’	22	23	21
59	FaiI	YATR	4	blunt	23	25	23
60	PleI	GAGTC	5	5’	23	21	21
61	TspDTI	ATGAA	5	3’	25	24	24
62	TspEI	AATT	4	5’	35	35	35
63	TfiI	GAWTC	5	5’	52	51	51
64	TaqI	TCGA	4	5’	66	62	62
65	HinfI	GANTC	4	5’	75	72	72

The loss or gains of the restriction sites with respect to the reference sequence are highlighted in bold.