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. 2013 Apr 18;11:50. doi: 10.1186/1741-7007-11-50

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Copyright © 2013 Chancerel et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Illustration of loci giving inconsistent results in the two mapping populations studied (F2 and G2): A, B, C, D polymorphic versus failed; E, F, G, H monomorphic versus failed. Counts for each class are available in Additional file 1. x-axis (norm Theta; normalized Theta) is ((2Π)Tan^-1(Cy5/Cy3)). Values close to 0 indicate homozygosity for one allele and values close to 1 indicate homozygosity for the alternative allele. y-axis (NormR; Normalized R) is the normalized sum of intensities for the two dyes (Cy3 ad Cy5).