Table 1.

Description of embryonic and larval stages used for the transcriptomic and amino acid content analyses

	Group	Group abbreviation	Developmental stages	Size (length or weight)	External morphological features
Embryos	Early embryos	EE	0-15	≤ 400 μm	*No visible eyes *Very slight body pigmentation
	Intermediate embryos	IE	16-18	400-800 μm	*Developing eye spots in many individuals *Pigmented bodies
	Late embryos	LE	19-20	> 800 μm	*Developed eye spots in all individuals *Highly pigmented bodies
Larvae	First instar larvae	L1	1st larval	≤ 0.2 mg	0-24 hours old
		L1 early			≤ 6 hours old
		L1 late			≥ 15 hours old

Assignment of embryos to the three groups (EE, IE or LE) was based on size range and on morphological criteria detailed in this table (see Figure 1A for exemplary microphotographs). The developmental stages were defined according to Miura et al.[13]. Larval stages were determined according to the development time after laying.