Skip to main content
. 2013 Apr 12;6:96. doi: 10.1186/1756-3305-6-96

Table 2.

Differentially expressed proteins between T. gondii-infected and non-infected mouse brain tissues

Spot No. Accession No. Protein name Score Expect Queries matched Sequence Coverage Nominal mass Calculated pI value Matched peptides Fold change (Infected/non-infected)A Functional categoriesB
Day 7, down-regulated proteins
1
IPI00230394
Lamin-B1
288
9.00E-25
27
36
66973
5.11
ASAPATPLSPTR;LQEKEELR;SLETENSALQLQVTEREEVR;ALYETELADARR;LREYEAALNSK;VDLENRCQSLTEDLEFRKNMYEEEINETRR;LVEVDSGR;LAQALHEMREQHDAQVRLYKEELEQTYHAK;LALDMEISAYR;LKNTSEQDQPMGGWEMIR;NQNSWGTGEDVK;NSQGEEVAQR;TTIPEEEEEEEEEPIGVAVEEERFHQQGAPR
−2.91
Structural molecule activity
2
IPI00944143
Serpin B6 isoform a
218
9.00E-18
9
25
45201
5.99
SRPGCCAGPRGYR;NVFLSPMSISSALAMVFMGAK;TGTQYLLR;FYEAELEELDFQGATEESR;FIEWTR;LGMTDAFGGR;AFVEVNEEGTEAAAATAGMMTVR
−1.68
Enzyme regulator activity and metabolic process
3
IPI00110721
Isoform 1 of Glyoxalase domain-containing protein 4
508
9.00E-47
12
41
33581
5.28
FQTVHFFRDVLGMQVLRHEEFEEGCK;LGNDFMGITLASSQAVSNAR;VAEGIFETEAPGGYKFYLQDR;IYEQDEEKQR;LELQGIQGAVDHAAAFGR;ELPDLEDLMKR;LLDDAMEADKSDEWFATR
−1.51
Cell part and organelle
4
IPI00113660
Proteasome activator complex subunit 3
471
4.50E-43
9
42
29602
5.69
ITSEAEDLVANFFPK;RLDECEEAFQGTK;SNQQLVDIIEKVKPEIR;IEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITR;YPHVEDYRRTVTEIDEKEYISLR
−1.70
Enzyme regulator activity
5
IPI00130344
Chloride intracellular channel protein 1
202
3.60E-16
6
39
27338
5.09
IGNCPFSQR;YPKLAALNPESNTSGLDIFAK;VLDNYLTSPLPEEVDETSAEDEGISQR;GFTIPEAFR;YLSNAYAREEFASTCPDDEEIELAYEQVAR
−1.89
Transporter activity
6
IPI00121851
Prefoldin subunit 3
133
2.90E-09
4
15
22592
6
FLLADNLYCK;NLDSLEEDLDFLR;VYNWDVKR
−1.51
Metabolic process
7
IPI00315794
Cytochrome b5
212
3.60E-17
6
54
16365
4.79
VEGSEPSVTYYR;NSAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAR;QYYIGDVHPSDLKPK
−1.70
Binding and enzyme activator activity
8
IPI00124501
Galectin-related protein A
257
1.10E-21
8
57
19172
5.12
LDDGHLNNSLGSPVQADVYFPRLIVPFCGHIK;AVFTDR;NSCISGERGEEQSAIPYFPFIPDQPFRVEILCEHPR;VFVDGHQLFDFYHRIQTLSAIDTIK
−1.74
Binding
9
IPI00308984
Eukaryotic translation initiation factor 1A
248
9.00E-21
3
28
16564
5.07
ELVFKEDGQEYAQVIK;LEAMCFDGVKR;VWINTSDIILVGLR
−1.67
Binding
Day 7, up-regulated proteins
10
IPI00131830
Serine protease inhibitor A3K
470
5.70E-43
9
28
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR
2.55
Enzyme regulator activity
11
IPI00131830
Serine protease inhibitor A3K
511
4.50E-47
12
35
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETK;AVLDVAETGTEAAAATGVIGGIR
3.15
Enzyme regulator activity
12
IPI00131830
Serine protease inhibitor A3K
463
2.90E-42
10
28
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR
6.30
Enzyme regulator activity
13
IPI00131830
Serine protease inhibitor A3K
564
2.30E-52
14
35
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETKK;AVLDVAETGTEAAAATGVIGGIR
4.02
Enzyme regulator activity
14
IPI00131830
Serine protease inhibitor A3K
596
1.40E-55
12
35
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETK;AVLDVAETGTEAAAATGVIGGIR
5.80
Enzyme regulator activity
15
IPI00310972
GTP-GDP dissociation stimulator 1 isoform b
490
5.70E-45
18
27
61381
5.28
NEFMR;DQEVLLQTGR;EQFASTNIAEELVK;QIEHDKREMIFEVLAPLAENDAIK;LMDLLDRHVEDGNVTVQHAALSALR;SEMPPVQFK;MLIDAQAEAAEQLGK;LVEWCEAKDHAGVMGESNR;DLASAQLVQILHRLLADER;SVAQQASLTEQR
8.32
Cell part and organelle part
Day 14, down-regulated proteins
16
IPI00131871
COP9 signalosome complex subunit 4
449
7.20E-41
18
50
46541
5.57
QLLTDFCTHLPNLPDSTAK;VISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLK;LYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYK;KFIEAAQR;TIVHESERLEALKHALHCTILASAGQQR;MLATLFKDERCQQLAAYGILEK;ATTADGSSILDR;IASQMITEGRMNGFIDQIDGIVHFETR
−2.50
Metabolic process
17
IPI00338039
Tubulin beta-2A chain
591
4.50E-55
22
42
50274
4.78
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNK;AILVDLEPGTMDSVR;GHYTEGAELVDSVLDVVRK;IREEYPDRIMNTFSVMPSPK;FPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPR;ISEQFTAMFRR
−1.90
Structural molecule activity
18
IPI00113660
Proteasome activator complex subunit 3
450
5.70E-41
11
43
29602
5.69
ERITSEAEDLVANFFPK;RLDECEEAFQGTK;SNQQLVDIIEKVKPEIR;IEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITR;YPHVEDYRRTVTEIDEKEYISLR
−2.04
Enzyme regulator activity
19
IPI00407019
Tetratricopeptide repeat protein 19
348
9.00E-31
12
23
41550
5.87
LSIMKDEPEAAELILHDALRLAYESDNRK;GQLENAEQLFK;QLSQAQR;ALQICQEIQGER;EIYQEALKR;RDEVSVQHIREELAELSR
−1.99
Cell part, organelle and organelle part
20
IPI00133440
Prohibitin
688
9.00E-65
14
42
29859
5.57
AVIFDRFR;DLQNVNITLRILFRPVASQLPRIYTSIGEDYDERVLPSITTEILK;FDAGELITQR;QVSDDLTER;QVAQQEAER;AAELIANSLATAGDGLIELRKLEAAEDIAYQLSR
−4.73
Metabolic process
21
IPI00313962
Ubiquitin carboxyl-terminal hydrolase isozyme L1
340
5.70E-30
8
39
25165
5.14
LGVAGQWR;QIEELKGQEVSPK;QFLSETEKLSPEDR;CFEKNEAIQAAHDSVAQEGQCR;MPFPVNHGASSEDSLLQDAAK;EFTEREQGEVR
−1.68
Cell proliferation
22
IPI00322312
Rho GDP-dissociation inhibitor 1
536
1.40E-49
12
49
23450
5.12
SIQEIQELDKDDESLR;YKEALLGRVAVSADPNVPNVIVTR;EGVEYR;YIQHTYR;IDKTDYMVGSYGPRAEEYEFLTPMEEAPK;FTDDDKTDHLSWEWNLTIK
−4.10
Enzyme regulator activity
23
IPI00845712
Isoform 1 of UPF0424 protein C1orf128 homolog
540
5.70E-50
17
73
24405
5.48
CAAEREEPPEQRGLAYGLYLR;LQCLNESR;GVFKPWEER;FVESDADEELLFNIPFTGNVKLKGVIIMGEDDDSHPSEMR;NIPQMSFDDTEREPEQTFSLNRDITGELEYATK;IFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFIS;
−2.40
Cell part, organelle and organelle part
24
IPI00221826
Isoform Short of Splicing factor, arginine/serine-rich 3
226
1.40E-18
7
41
14422
10.12
DSCPLDCK;AFGYYGPLRSVWVARNPPGFAFVEFEDPR;NRGPPPSWGR;DDYRR
−1000000
Metabolic process
Day 14, up-regulated proteins
25
IPI00123613
Protein kinase C and casein kinase substrate in neurons protein 1
382
3.60E-34
15
40
50886
5.15
LCNDLMSCVQER;AYAQQLTDWAKR;AWGAMMTEADKVSELHQEVK;AYHLACKEER;TTPQYMEGMEQVFEQCQQFEEKR;HLNLAENSSYMHVYRELEQAIRGADAQEDLR;KAEGATLSNATGAVESTSQAGDR;ALYDYDGQEQDELSFK;LGEEDEQGWCRGRLDSGQLGLYPANYVEAI
4.65
Catalytic activity and binding
26
IPI00110265
Isoform 1 of NAD-dependent deacetylase sirtuin-2
255
1.80E-21
11
35
43856
5.23
NLFTQTLGLGSQK;LLDELTLEGVTRYMQSER;VICLVGAGISTSAGIPDFR;HPEPFFALAK;CYTQNIDTLER;IFSEATPRCEQCQSVVKPDIVFFGENLPSR;APLATPR;ELEDLVRREHANIDAQSGSQAPNPSTTISPGK
1.57
Catalytic activity and binding
27
IPI00649438
Novel protein similar to splicing factor, arginine/serine-rich 3
80
0.00061
3
19
19404
11.41
NPPGFAFVEFEDPRDAADAVR;NRGLPPSWGR
1.65
Metabolic process
Day 21, down-regulated proteins
28
IPI00133522
Protein disulfide-isomerase
408
9.00E-37
17
37
57507
4.79
SNFEEALAAHK;ALAPEYAKR;LKAEGSEIR;VDATEESDLAQQYGVR;YQLDKDGVVLFKKFDEGRNNFEGEITK;HNQLPLVIEFTEQTAPK;ILFIFIDSDHTDNQR;KEECPAVR;YKPESDELTAEKITEFCHR;IKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKK;LGETYKDHENIIIAK;FFPASADR
−1.52
Metabolic process
29
IPI00756786
SH3-domain GRB2-like endophilin B2
258
9.00E-22
11
26
45165
5.44
KLASDAGIFFTR;FGQAEKTELDAHFENLLAR;NWTER;QTEVLLQPNPSAR;ATCEGDTVPDFQETRPR;VAQTEFDRQAEVTRLLLEGISSTHVNHLR;SQTTYYAQCYR
−1.50
Cellular process
30
IPI00111556
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
239
7.20E-20
10
34
36123
5.21
SPDTNYLFMGDYVDR;QITQVYGFYDECLRKYGNANVWK;LQEVPHEGPMCDLLWSDPDDRGGWGISPR;AHQLVMEGYNWCHDRNVVTIFSAPNYCYR;YSFLQFDPAPR
−2.10
Metabolic process
31
IPI00798544
Isoform 2 of Enolase-phosphatase E1
384
2.30E-34
11
24
25457
4.92
QLQGHMWK;MKAEFFADVVPAVRRWREAGMKVYIYSSGSVEAQK;GHKVDSESYRK
−3.54
Metabolic process
32
IPI00230145
Ferritin heavy chain
408
9.00E-37
11
65
21224
5.53
QNYHQDAEAAINR;YFLHQSHEEREHAEK;KPDRDDWESGLNAMECALHLEKSVNQSLLELHKLATDKNDPHLCDFIETYYLSEQVK;ELGDHVTNLRKMGAPEAGMAEYLFDKHTLGHGDES
−1.56
Immune system process
Day 21, up-regulated proteins
33
IPI00403810
Tubulin alpha-1C chain
174
2.30E-13
5
16
50562
4.96
AVFVDLEPTVIDEVR;EIIDLVLDR;NLDIERPTYTNLNR;FDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
1.56
Structural molecule activity
34
IPI00221540
Erlin-2
339
7.20E-30
8
17
38077
5.37
SVQTTLQTDEVK;VTKPNIPEAIRR;VAQVAEITYGQK;KISEIEDAAFLAR;AKADAECYTALK
1.85
Metabolic process
35
IPI00230192
Isoform Alpha-1 of Guanine nucleotide-binding protein G(o) subunit alpha
170
5.70E-13
5
16
40629
5.34
AMDTLGVEYGDKER;LWGDSGIQECFNR;YYLDSLDRIGAGDYQPTEQDILR;LFDVGGQR
1.54
Signal transducer activity
36
IPI00653664
Isoform 1 of Enolase-phosphatase E1
365
1.80E-32
11
27
28696
4.79
EYLQTHWEEEECQQDVSLLR;QLQGHMWK;MKAEFFADVVPAVRRWREAGMKVYIYSSGSVEAQK;VDSESYRK
1.96
Metabolic process
37
IPI00130280
ATP synthase subunit alpha
376
1.40E-33
11
18
59830
9.22
TGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIAR;NVQAEEMVEFSSGLK;TGAIVDVPVGEELLGR;APGIIPRISVREPMQTGIKAVDSLVPIGR;ELI IGDR
2.43
Transporter activity and developmental process
38
IPI00129161
Somatotropin
201
4.50E-16
14
45
24986
5.97
AQHLHQLAADTYKEFERAYIPEGQR;EEAQQRTDMELLR;IFTNSLMFGTSDR;LKDLEEGIQALMQELEDGSPR;QTYDKFDANMR;AETYLR;RFVESSCAF
19.73
Cell proliferation
Spots 39–46 were down-regulated on both day 14 and day 21
39
IPI00119113
V-type proton ATPase subunit B
641
4.50E-60
34
64
56857
5.57
MALRAMRGIVNGAAPELPVPTGGPMAGAR;NYLSQPR;YAEIVHLTLPDGTKR;AVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGR;GPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIAR;IPIFSAAGLPHNEIAAQICR;SKDVVDYSEENFAIVFAAMGVNMETAR;LALTTAEFLAYQCEKHVLVILTDMSSYAEALR;RGFPGYMYTDLATIYER;QIYPPINVLPSLSRLMKSAIGEGMTR;DHADVSNQLYACYAIGK;AVVGEEALTSDDLLYLEFLQK;NFITQGPYENRTVYETLDIGWQLLR;RIPQSTLSEFYPR
−2.11
Transporter activity
40
IPI00759870
Isoform 4 of Heterogeneous nuclear ribonucleoproteins C1/C2
201
4.50E-16
3
9
32261
4.95
GFAFVQYVNER;MYSYPARVPPPPPIAR
−1.52
Metabolic process
41
IPI00115117
Stomatin-like protein 2
231
4.50E-19
12
32
38475
8.95
NTVILFVPQQEAWVVER;ILEPGLNVLIPVLDR;ASYGVEDPEYAVTQLAQTTMR;YEIKDIHVPPRVKESMQMQVEAER;ATVLESEGTR;APVPGAQNSSQSRRDVQATDTSIEELGR
−2.33
Organelle and organelle part
42
IPI00830393
Putative uncharacterized protein Cops6
209
7.20E-17
8
31
33855
5.65
SQEGRPMQVIGALIGK;NIEVMNSFELLSHTVEEK;QVCEIIESPLFLK;IGVDHVAR;LILEYVKASEAGEVPFNHEILR;TCNTMNQFVNKFNVLYDR
−2.91
Cellular process and metabolic process
43
IPI00311515
Beta-soluble NSF attachment protein
415
1.80E-37
20
69
33878
5.32
EAVQLMAEAEKR;ASHSFLRGLFGGNTRIEEACEMYTR;NWSAAGNAFCQAAK;HDSATSFVDAGNAYK;HHITIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANK;VAAYAAQLEQYQKAIEIYEQVGANTMDNPLLK;AALCHFIVDELNAKLALEKYEEMFPAFTDSRLLEAHEEQNSEAYTEAVKEFDSISRLDQWLTTMLLR;
−1.67
Cellular process
44
IPI00322312
Rho GDP-dissociation inhibitor 1
396
1.40E-35
9
42
23450
5.12
SIQEIQELDKDDESLR;YKEALLGRVAVSADPNVPNVIVTR;EGVEYR;YIQHTYR;IDKTDYMVGSYGPR;FTDDDKTDHLSWEWNLTIK
−1000000
Enzyme regulator activity
45
IPI00133853
UPF0687 protein C20orf27 homolog
268
9.00E-23
8
50
19692
6.19
FAAGHDAEGSQSHVHFDEK;YEITFTLPPVR;ETPVHSLHLKLLSVTPTSEGYSIK;EGVLKEEMLLACEGDIGTCVR;HHGTPMLLDGVK
−1.70
Cell part and organelle part
46
IPI00315504
ADP-ribosylation factor-like protein 2
75
0.0016
2
8
21022
5.67
ELQSLLVEER;SHHWR
−1.66
Binding
Spots 47–56 were up-regulated on both day 14 and day 21
47
IPI00129526
Endoplasmin
766
1.40E-72
33
38
92703
4.74
TDDEVVQREEEAIQLDGLNASQIR;FAFQAEVNR;NKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVK;NLLHVTDTGVGMTREELVKEVEEDEYKAFYK;ESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKK;RVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSR;IADEKYNDTFWK;LGVIEDHSNR;FQSSHHSTDITSLDQYVER;QDKIYFMAGSSRKEAESSPFVEREATEKEFEPLLNWMK;LTESPCALVASQYGWSGNMER;DISTNYYASQKKTFEINPR;AYGDRIER
1.54
Metabolic process
48
IPI00123639
Calreticulin
292
3.60E-25
5
16
48136
4.33
EQFLDGDAWTNR;HEQNIDCGGGYVK;IDNSQVESGSLEDDWDFLPPKKIKDPDAAKPEDWDER;GEWKPR
1.66
Immune system process,
49
IPI00131830
Serine protease inhibitor A3K
175
1.80E-13
6
21
47021
5.05
ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;AVLDVAETGTEAAAATGVIGGIR;
1000000
Enzyme regulator activity
50
IPI00131830
Serine protease inhibitor A3K
336
1.40E-29
9
27
47021
5.05
GKTMEEILEGLK;DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;AVLDVAETGTEAAAATGVIGGIR
12.57
Enzyme regulator activity
51
IPI00131830
Serine protease inhibitor A3K
316
1.40E-27
8
26
47021
5.05
DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR
5.29
Enzyme regulator activity
52
IPI00131830
Serine protease inhibitor A3K
187
1.10E-14
5
16
47021
5.05
ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLR;AVLDVAETGTEAAAATGVIGGIR
1000000
Enzyme regulator activity
53
IPI00131287
Isoform 1 of N-terminal EF-hand calcium-binding protein 2
374
2.30E-33
11
28
43870
5.18
APLVPDIPSADPGPGPAASRGGTAVILDIFR;KVYEGGSNVDQFVTR;YGGPTPPYIPNHK;TLSFDLQQRLSDEEGTNMHLQLVRQEMAVCPEQLSEFLDSLR;NCFHVAAVR
1000000
Cell part and binding
54
IPI00928020
Putative uncharacterized protein Otub1
331
4.50E-29
7
25
28190
5.15
IQQEIAVQNPLVSER;EYAEDDNIYQQK;YSYIRKTRPDGNCFYR;LLTSGYLQR;FFEHFIEGGR
1.89
Metabolic process
55
IPI00323571
Apolipoprotein E
329
7.20E-29
14
39
35901
5.56
FWDYLR;ELEEQLGPVAEETR;LGADMEDLR;NEVHTMLGQSTEEIR;LSTHLR;LGPLVEQGR;TANLGAGAAQPLR;AQAFGDR;GRLEEVGNQAR;SKMEEQTQQIRLQAEIFQAR;GWFEPIVEDMHR
1000000
Binding and antioxidant activity,
56 IPI00278230 NFU1 iron-sulfur cluster scaffold homolog 131 4.50E-09 5 17 28922 4.92 FIPGKPVLETRTMDFPTPAAAFR;IRPTVQEDGGDVIYRGFEDGIVR 1000000 Organelle and organelle part