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. 2013 May 4;10:23. doi: 10.1186/1742-9994-10-23

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Copyright © 2013 Moeller et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Agglutination and lysis capacities of free-ranging Gila monsters. Agglutination and lysis scores as a function of (A, B) plasma osmolality, (C, D) month of sampling, and (E, F) change in tail volume in free-ranging Gila monsters. Individuals with a greater plasma osmolality (i.e., more dehydrated) had greater agglutination and lysis scores. Change in tail volume (a measure of energy balance) was negatively related to lysis (F_1,21 = 7.74, P = 0.011), but after removing a gravid female from analysis, this was non-significant (F_1,18 = 3.76, P = 0.068), a change denoted with a dashed line. Change in tail volume had no significant relation (F_1,21 = 3.61, P = 0.071) to agglutination. There was no effect of month on either agglutination or lysis ability (all P > 0.05). No individual was disproportionately influential (all Cook’s D < 4/n) in these analyses. Raw data are presented, uncorrected for individual. LSMeans with error bars (SE) from the mixed model are presented for month of sampling.