Table 1.

Sources, pre-treatments and dilutions of the antibodies used in this study

Antibody	Source	Clone	Pre-treatment	Dilution
Anti-oestrogen receptor	Zymed / Life Technologies, USA	Poly rabbit	Nuclear Decloaker. Biocare Medical. Concord. CA. USA. pH 9.5. 3× 5 min.	1:200
Anti-progesterone receptor	Immunotech SAS, Marseille, France	10A9 rabbit	Boil in citrate buffer, pH 6.0, 4× 5 min.	1:200
Anti-Ki-67	Thermo Fisher Scientific, Fremont, CA, USA	SP6 rabbit	Boil in citrate buffer, pH 6.0, 3× 5 min.	1:400
Anti-cytokeratin	Sigma-Aldrich, Germany	8.13 mouse	Boil in citrate buffer, pH 6.0, 2× 5 min.	1:100
Anti-vimentin	Dako, Glostrup, Denmark	V9 mouse	No pre-treatment	1:400
Anti-laminin	Dako, Glostrup, Denmark	Poly rabbit	Protease (Sigma) 1mg/mL PBS, 20 min, room temp.	1:500
Anti-β-catenin	Acris Antibodies, Herford, Germany	9G2 mouse	Boil in citrate buffer, pH 6.0, 3× 5 min.	1:100