Figure 2. Anti-angiogenic activity of the methanolic extract of Rhynchosia viscosa.

Inhibition of vascular outgrowth was determined in fli-1:EGFP transgenic embryos. At 16 hours post-fertilization (hpf), embryos were incubated with different concentrations of the methanolic extract of the plant and anti-angiogenic effects were assessed at 48 hpf. A to C, all embryos are 48 hpf, with anterior to the left, scale bar = 10 µm. A, untreated control (DMSO 1%); B, zoom of A (dashed box) showing normal outgrowth of intersegmental vessels (ISV) along the trunk of the larva (arrows); C, embryo treated with 50 µg/mL crude methanolic extract of R. viscosa. Inhibition or reduction of ISV growth is observed along the trunk (arrows); D, IC₅₀ curve and values showing the inhibitory activity of the methanolic extract of R. viscosa.