Figure 5. Bioactive compounds of Rhynchosia viscosa in the vascular outgrowth assay.

IC₅₀ curves and values were determined for each of the bioactive constituents of the methanolic extract of R. viscosa. Each compound, at six different concentrations, was assessed for their effect in the inhibition of intersegmental vessel (ISV) growth. A to F, all embryos are 48 hours post-fertilization (hpf), with anterior to the left, scale bar = 10 µm. A, untreated control (DMSO 1%); B, zoom of A (dashed box) showing normal outgrowth of intersegmental vessels (ISV) along the trunk of the larva (arrows); C, embryo treated with 50 µM genistein; D, embryo treated with 100 µM rhynchoviscin; E, embryo treated with 50 µM licoisoflavone A; F, embryo treated with 50 µM sophoraisoflavone. Arrowheads point the interconnection zone between the dorsal aorta and the posterior cardinal vein, arrows point the ISV; G, IC₅₀ curves and values (µM) for each of the bioactive compounds of R. viscosa.