Table 1. Antigene PNA sequences targeting highly conserved sequences in the NS gene of swine-origin influenza A/H1N1 and the HA gene of human pathogenic influenza A/H3N2.
PNA sequence (N to C) | Mass | ||
Calcd. | Found | ||
Anti-H1N1(NS) PNA | |||
PNA 1 | H-(Lys)3-AEEA-CCTTCTCTTCCAGGA-Lys-(AEEA)2-Lys-NH2 | 4682.14 | 4682.18 |
PNA 2 | H-(Lys)3-TCTCTTCC-AEEA-CCTTCTCTTCCAGGA-Lys-(AEEA)2-Lys-NH2 | 7136.88 | 7137.13 |
PNA 3 | H-(Lys)3-TCTCTTCC-AZO-CCTTCTCTTCCAGGA-Lys-(AEEA)2-Lys-NH2 | 7275.09 | 7299.75 |
PNA 4 | H-(Lys)3-TCTATTCC-AZO-CCTTATCTTCAAGGA-Lys-(AEEA)2-Lys-NH2 | 7347.15 | 7378.55 |
Anti-H3N2(HA) PNA | |||
PNA 5 | H-(Lys)3-TTCCCTCC-AEEA-CCTCCCTTAGGTCAC-Lys-(AEEA)2-Lys-NH2 | 7106.98 | 7106.27 |
PNA 6 | H-(Lys)3- TTCCCTCC-AZO-CCTCCCTTAGGTCAC-Lys-(AEEA)2-Lys-NH2 | 7245.19 | 7246.93 |
AEEA: 2-aminoethoxy-2-ethoxy acetic acid, AZO: thioazobenzene, Lys: lysine.