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. 2013 May 21;8(5):e63919. doi: 10.1371/journal.pone.0063919

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© 2013 Dhar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) SDS–PAGE of recombinant YeSodA and YeSodB expressed in pET 28a (+) (samples were resolved on 15% polyacrylamide gel and stained with Coomassie Brilliant Blue R-250). The purified SodA and SodB showed a single band each of 23 KDa and 21 kDa respectively. M1 and M2: Protein marker; Lane 1: SodA; Lane 2 SodB. (b) Molecular weight determination of YeSodA (82 kDa) and YeSodB (21 kDa) by Sephacryl S-200 molecular sieve chromatography. The molecular weight of marker proteins (SigmaAldrich) were as follows: β-Amylase (200 kDa), Alcohol dehydrogenase (150 kDa), BSA (66 kDa), Carbonic anhydrase (29 kDa) and Cytochrome C (12.4 kDa). (c) Zymogram analysis showing achromatic bands of YeSodA and YeSodB against a dark background. Lane 1: YeSodA; Lane 2: YeSodB. (d) Isoelectric point (pI) of purified recombinant YeSodA and YeSodB stained with coomassie brilliant blue. M: pI marker; Lane 1: YeSodA; Lane 2: YeSodB.