Table 2. Details of primers and the PCR conditions.

Gene	Primers	Sequence (5'-3')	Amplicon(bp)	Annealing temperature	Restrictionsites	Position of primers on the Y.e. strain 8081 complete genome
sodA	SodA 624F	TTTGGATCCATGAGTTACTCACTGCCATCCCT	624	58°C –40s	BamHI and SacI	4530100–4530122
	SodA 624R	TTTGAGCTCCTTAGCTTGAGCGAAGCGC				4530720–4530702
sodB	SodB 579F	TTTGAGCTCATGTCTTTTGAATTACCGGCGTT	579	59°C –40s	SacI and NotI	2356988–2357007
	SodB 579R	TTTGCGGCCGCCCGGCTAAGTTTTTCTCAACGAA				2357563–2357543
sodC	SodC 525F	AAAGCGGCCGCATGAAACTGAAATACTTAGTGTTACCC	525	50°C –40s	NotI and XhoI	877988–878014
	SodC 525R	AAACTCGAGTTACTCAATCACACCACAAGC				878514–878492
sodC	SodC Trun F	AAAGCGGCCGCATGGCTGCGGATATTACTGTGAC	476	50°C –40s	NotI and XhoI	NA
	SodC 524R	AAACTCGAGTTACTCAATCACACCACAAGC				878514–878492

Nucleotide bases in bold represent restriction sites. NA: the primer was designed using the sequence of sodC (accession no. JX204785) from strain IP27366 as the template.