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. 2013 May 21;8(5):e63435. doi: 10.1371/journal.pone.0063435

Figure 2. Fenofibrate inhibits LPS-mediated pro-inflammatory cytokine generation by induction of SHP.

Figure 2

BMDMs from Shp +/+ and Shp −/− mice were pretreated with fenofibrate (50 µM) for 4 h and then stimulated with LPS (100 ng/mL) for 6 h (A), 18 h (B), or 48 h (C). (A) Semi-quantitative RT-PCR analyses of Tnfα, Il6, Il10, shp, and Actb mRNA expression. (B) ELISA analyses of TNF-α, IL-6, IL-8, and IL-10 proteins in culture supernatants. (C) Immunoblot analysis of HMGB1 protein in whole cell lysate (WCL) and supernatant (SN). (D) ELISA analyses of TNF-α, IL-6, IL-8, and IL-10 proteins in culture supernatants. BMDMs from WT mice were transduced with Ad-GFP, Ad-SHP, or Ad-siSHP (MOI = 10) for 48 h, followed by treatment with fenofibrate (50 µM) for 4 h prior to LPS (100 ng/mL) stimulation. Left, Semi-quantitative RT-PCR analyses of the efficiency of adenoviral transduction. The data (A, C, and D left) are representative of at least three independent experiments with similar results. Quantitative data are shown as the mean ± SD of three experiments (B, and D right). Statistical differences (**, p<0.01; ***, p<0.001), as compared to the control mice, are indicated (paired t-test with Bonferroni adjustment). FF, fenofibrate; n.s., non-specific.