Figure 6. JQ1 treatment suppresses c-Myc expression and function and reduces ligand-independent prostate cancer cell survival.

LNCaP, abl, and 22RV1 cells were grown in charcoal-stripped serum and treated with vehicle, 50 nM, 250 nM or 500 nM JQ1 every 24 hours for 72 hours. A) Immunoblotting was performed to determine the protein levels of c-Myc. B) QRT-PCR was performed to determine the mRNA level of c-Myc and c-Myc targets genes KIF11, CDKN1A, TPX2, and AURKB relative to actin. *denotes p<0.05 compared to vehicle. C) Cell viability was determined at the end of treatment with the trypan blue exclusion method. p<0.01 for the 250 nM and 500 nM doses vs. vehicle in all three cell lines.