Figure 3.

Lung histology. A- Healthy mice (control). B-Lung fibrosis was induced by intratracheal instillation of 1.5 U/kg of bleomycin. C-Mice were treated with inhalation of liposomal PGE2 within 3 weeks twice a week starting one day later after the bleomycin administration. At the end of experiment, lungs were harvested and fixed in 10% phosphate-buffered formalin. Samples were subsequently dehydrated and embedded in Paraplast®. Sections (5 μm) were cut, stained with hematoxylin and eosin. Representative images are shown (10X magnification).