Table 2.
Proteins in skeletal muscle differing significantly among groups
| Protein name | NSAF | Number of subjects with protein assigned | Total spectra per group | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| Control | T2D Pre | T2D Post | Control | T2D Pre | T2D Post | Control | T2D Pre | T2D Post | |
| GOT2 Aspartate aminotransferase | 31.8 ± 2.4 | 22.8 ± 1.7* | 27.2 ± 2.4# | 6 | 6 | 6 | 530 | 390 | 476 |
| MYH1 Myosin-1 | 25.2 ± 10 | 68.1 ± 11.3* | 55.2 ± 7.3* | 4 | 6 | 6 | 1980 | 4811 | 3976 |
| UCRC Cytochrome b-c1 complex subunit 9 | 19.8 ± 1.3 | 8.9 ± 4.0* | 15.4 ± 3.9 | 6 | 3 | 5 | 48 | 26 | 40 |
| DCXR L-xylulose reductase | 3.0 ± 0.4 | 1.2 ± 0.6* | 1.3 ± 0.6 | 6 | 3 | 3 | 29 | 13 | 15 |
| MT-CO2 Cytochrome c oxidase subunit 2 | 22.2 ± 1.8 | 16.4 ± 1.4* | 18.0 ± 2.4 | 6 | 6 | 6 | 193 | 145 | 158 |
| AHSG cDNA FLJ55606, highly similar to Alpha- 2-HS-glycoprotein | 1.0 ± 0.5 | 3.0 ± 0.7* | 2.8 ± 0.5* | 3 | 6 | 6 | 17 | 43 | 41 |
| MDH2 Malate dehydrogenase | 58.1 ± 3.8 | 46.9 ± 2.5* | 53.5 ± 4.4 | 6 | 6 | 6 | 753 | 623 | 697 |
| ATP5A1 ATP synthase subunit α | 79.2 ± 6.3 | 61.2 ± 3.6* | 67.0 ± 4.8 | 6 | 6 | 6 | 1705 | 1309 | 1468 |
| GOT1 Aspartate aminotransferase, cytoplasmic | 40.0 ± 3.9 | 29.8 ± 1.5* | 36.1 ± 2.9# | 6 | 6 | 6 | 648 | 471 | 589 |
| AKR1B1 Aldose reductase | 15.1 ± 0.9 | 12.0 ± 1.0* | 11.7 ± 1.0* | 6 | 6 | 6 | 183 | 147 | 142 |
| TF Serotransferrin | 7.1 ± 1.5 | 12.9 ± 1.9* | 12.1 ± 2.9 | 6 | 6 | 6 | 194 | 325 | 328 |
| ATP5F1 ATP synthase subunit b | 25.6 ± 1.9 | 18.6 ± 2.3* | 18.2 ± 4.1 | 6 | 6 | 6 | 255 | 189 | 187 |
| PHB2 Prohibitin-2 | 12.88 ± 1.63 | 8.07 ± 1.30* | 9.68 ± 1.68 | 6 | 6 | 6 | 148 | 95 | 116 |
| GPD1L Glycerol-3- phosphate dehydrogenase 1-like protein | 4.7 ± 0.5 | 3.3 ± 0.4* | 3.6 ± 0.3* | 6 | 6 | 6 | 62 | 44 | 48 |
| ECH1 Delta(3,5)- Delta(2,4)-dienoyl-CoA isomerase | 16.1 ± 1.5 | 11.4 ± 1.4* | 15.2 ± 2.0 | 6 | 6 | 6 | 201 | 145 | 195 |
| CKMT2 Creatine kinase | 29.4 ± 2.0 | 26.7 ± 3.1 | 38.3 ± 4.4# | 6 | 6 | 6 | 476 | 454 | 668 |
| LDHB Lactate dehydrogenase B chain | 20.9 ± 5.0 | 10.2 ± 2.8 | 15.4 ± 3.1# | 6 | 6 | 6 | 259 | 121 | 191 |
| ANXA2 Annexin A2 | 16.9 ± 2.8 | 13.9 ± 1.0 | 17.3 ± 1.7# | 6 | 6 | 6 | 232 | 187 | 233 |
| UQCRC2 Cytochrome b- c1 complex subunit 2 SUCLA2 Isoform 1 of | 22.2 ± 2.1 | 20.0 ± 2.09 | 23.9 ± 1.9# | 6 | 6 | 6 | 390 | 365 | 439 |
| Succinyl-CoA ligase [ADP-forming] subunit beta | 4.0 ± 1.0 | 3.1 ± 0.8 | 4.9 ± 1.0# | 5 | 5 | 5 | 70 | 59 | 93 |
| PDLIM3 Isoform 1 of PDZ and LIM domain protein 3 | 24.9 ± 2.9 | 22.7 ± 2.9 | 17.8 ± 1.9# | 6 | 6 | 6 | 355 | 316 | 251 |
| PTGES2 Prostaglandin E synthase | 2.5 ± 0.8 | 1.4 ± 0.6 | 2.5 ± 0.6# | 4 | 4 | 5 | 38 | 24 | 41 |
| PFKM Isoform 1 of 6- phosphofructokinase EEF1G cDNA | 27.8 ± 3.9 | 36.3 ± 5.0 | 27.3 ± 4.2# | 6 | 6 | 6 | 834 | 1090 | 817 |
| FLJ56389, highly similar to Elongation factor 1-γ | 3.1 ± 0.4 | 2.3 ± 0.4 | 3.3 ± 0.5# | 6 | 6 | 6 | 55 | 41 | 57 |
| FH Isoform Cytoplasmic of Fumarate hydratase | 9.5 ± 0.8 | 8.3 ± 0.9 | 10.6 ± 0.7# | 6 | 6 | 6 | 180 | 161 | 206 |
| ALDOA Fructose- bisphosphate aldolase A | 256.9 ± 28.5 | 267.2 ± 22.0 | 234.9 ± 22.2# | 6 | 6 | 6 | 3510 | 3749 | 3320 |
| HADHA Trifunctional enzyme subunit α | 26.54 ± 1.68 | 21.15 ± 1.89 | 25.61 ± 2.44# | 6 | 6 | 6 | 774 | 632 | 757 |
| IDH2 Isocitrate dehydrogenase [NADP] | 53.0 ± 4.7 | 44.0 ± 3.9 | 54.4 ± 3.0# | 6 | 6 | 6 | 908 | 771 | 975 |
| GLO1 Lactoylglutathione lyase | 7.8 ± 1.5 | 9.7 ± 1.7 | 5.6 ± 2.0# | 6 | 6 | 6 | 58 | 73 | 44 |
| NDUFB8 NADH dehydrogenase (Ubiquinone) 1 beta subcomplex, 8 | 0.3 ± 0.3 | 1.3 ± 0.6 | 2.4 ± 0.5* | 1 | 5 | 5 | 5 | 10 | 17 |
| CRYAB Alpha-crystallin B chain | 149.5 ± 15.5 | 108.9 ± 9.8 | 91.0 ± 13.3* | 6 | 6 | 6 | 986 | 714 | 595 |
Data are given as Mean ± SEM for Normalized Spectral Abundance Factors (NSAF) × 104. NSAF is calculated as described in the text. Total spectra = sum of spectra across six subjects in each group.
*
P < 0.05 vs. Controls,
#
P < 0.05 pre vs. post training.