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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Immunol. 2013 May 1;190(11):5818–5828. doi: 10.4049/jimmunol.1203452

Figure 7.

Figure 7

Notch inhibition enhances the accumulation of natural Foxp3+ regulatory T cells after allo-BMT. (A Detection of live regulatory T cell (Tregs) on day 5 post-transplantation using Foxp3-IRES-RFP (FIR) mice crossed to ROSA26DNMAMLf×Cd4-Cre mice, showing expanded Tregs among Notch-deprived alloreactive T cells; (B) To determine the origin of the expanded Tregs, lethally irradiated BALB/c recipients were transplanted with WT or DNMAML CD4+ T cells with or without FIR+ Tregs and analyzed on day 14 post-transplantation; (C) Post-sort purity of WT and DNMAML CD4+ T cell fractions after depletion of FIR+ cells; (D) Frequency of donor-derived FoxP3-IRES-RFP+ T cells as assessed by flow cytometry after transplantation of WT or DNMAML CD4+ T cells, including Tregs (+Treg), or depleted of Tregs (-Treg). Day 14 alloreactive DNMAML Tregs were derived from preexisting Tregs. *p<0.05; *** p<0.001.