Figure 8.

Alloreactive Notch-deprived CD8⁺ T cells maintain high cytotoxic potential. (A) In vivo cytotoxicity assay. Transplanted BALB/c recipient mice were challenged on day 14 with a 1:1 mixture of CFSE-labeled allogeneic targets and control cells (CFSE^High H-2Kd⁺ BALB/c and CFSE^low control H-2Kb⁺ B6-CD45.1 splenocytes, respectively). After 18 hours, elimination of the BALB/c targets was assessed in the spleen by flow cytometry; (B) BALB/c recipients were transplanted with TCD BM and WT or DNMAML splenocytes (5×10⁶ each). On day 8, alloreactive WT and DNMAML and naïve CD8⁺ T cells were MACS-purified and incubated with ⁵¹Chromium-labeled tumor cells at various E:T ratios for 5 hours (representative of n=3 independent experiments). A20 and P815 cells were allogeneic targets (H2kd). EL4 cells were syngeneic controls (H2kb). **p<0.01; ***p<0.001.