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. 2013 May 21;8(5):e63295. doi: 10.1371/journal.pone.0063295

Figure 2. Necrosis was induced by ANE in serum-starved cells.

Figure 2

(A) SAS cells were treated with 0.3 mg/ml for 12 hours and stained with acridine orange (AO) and ethidium bromide (EtBr). (B) SAS cells treated with 1 mg/ml ANE for 24 hours were stained with Annexin V (green) and propidium iodide (red). Cells treated with 10 µg/ml puromycin were used as the positive control of apoptosis. (C) SAS and OC2 cells treated with 1 mg/ml ANE or 20 µg/ml puromycin were harvested for DNA fragmentation analysis 48 hours after treatment.