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. 2013 May 21;8(5):e63529. doi: 10.1371/journal.pone.0063529

Figure 8. (R)-(+)-Methanandamide induces the differentiation of GABAergic neurons and neuritogenesis.

Figure 8

A: Schematic representation of the protocol. B: Bar graph depicts the numbers of either VGAT- or TH/βIII tubulin-positive cells, expressed as percentage of total cells. The data are expressed as percentage ± SEM. N = 3. *P<0.05 using unpaired Student’s t test for comparison with control. C: Schematic representation of the protocol used for studying neuritogenesis. D: Representative confocal digital images of the GFP (green), MAP2 (red), Hoechst staining (blue), in control cultures and in cultures exposed to R-m-AEA. Scale bar = 20 µm. E: Bar graphs depict (from left to right): total length (µm), number of primary and number of secondary ramifications of MAP2 neurites per cell. N = 3. **P<0.01 using unpaired student’s t test for comparison with control. MAP2: Microtubule-associated protein 2; TH: tyrosine hydroxylase; βIII tubulin: Neuron-specific class III beta-tubulin; VGAT: vesicular GABA transporter.