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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Immunol. 2013 Apr 29;190(11):5588–5599. doi: 10.4049/jimmunol.1203216

Figure 2. Ag bound to B cells via CD19 is co-localized with the BCR and co-engagement of the BCR and CD19 activates B cells.

Figure 2

(A) Purified B cells were incubated with scFv-p3-4 fusion protein for 30 min and stained with biotinylated anti-her2/neu followed by Alexa Fluor 594-streptavidin (red) and Alexa Fluor 488-anti-IgM (green). Cells were cytospin to slides and images were acquired. (B) B cells were stimulated with fusion proteins as indicated for 24 h. Surface markers were assessed by flow cytometry. Data shown are one from 3 independent experiments. (C) Supernatants from B cells stimulated with fusion proteins scFv, p3-4, scFv-p3-4, or medium alone for 48 h were harvested and assayed for cytokines IL-6, IL-12p40, and TNF-α by ELISA. Representative of 3 experiments. Error bars represent s.e.m.