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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Immunol. 2013 Apr 29;190(11):5588–5599. doi: 10.4049/jimmunol.1203216

Figure 5. Increased IFN-γ-producing CD8 T cells, IL-4-producing CD4 T cells, T cell proliferation, and augmented in vivo cytolytic activity elicited by targeting of her-2/neu to B cells.

Figure 5

(A, C) BALB/c mice (n=3) were immunized with scFv, P3-4 or scFv-p3-4 for 4 times at a 1-week interval. Splenocytes from immunized mice were stimulated with scFv-p3-4 (20 μg/ml) for 3 days. Intracellular IFN-γ of CD4 and CD8 (A) was carried out and 3H-thymidine proliferation were measured (C). (B) IL-4 production. Cells from immunized mice (n=3-5) as described above were stimulated with ConA, IL-2, and IL-4 and then re-stimulated with immobilized CD3 and soluble CD28 mAbs. Intracellular IL-4 staining was performed. (D) In vivo cytotoxicity. CFSE labeled syngeneic B cells pulsed with (CFSE high) or without scFv-p3-4 (CFSE low) were injected intravenously into immunized mice. Mice were sacrificed 24 h after transfer and splenocytes were harvested and assessed by flow cytometry. Cells were gated on CFSE-positive cells. Representative histograms and summarized % of cytotoxicity were shown. Data are representative of 3 experiments.