Diazoxide inhibits succinate dehydrogenase (SDH) activity in wild type mice mitochondria. (A) Isolated mitochondria were exposed to test solutions for 20 minutes. SDH activity is represented as percent change in absorbance over time. Green line, succinate (20 mM) (control) (n=11); black line, succinate + DMSO (control) (n=10); blue line, malonate (8 mM) + succinate (n=11); red line, diazoxide (100 uM) + succinate (n=10). *p<0.05 vs succinate, DMSO; †p<0.0001 vs malonate. (B) SDH activity represented as the average slope. Green bar, succinate (20 mM) (control) (n=11); black bar, 1% DMSO + succinate (control) (n=10); blue bar, malonate (8 mM) + succinate (n=11); red bar, diazoxide (100 uM) + succinate (n=10). *p<0.05 vs succinate, DMSO; †p<0.05 vs malonate.