Table 1.
Antibodies used for immunohistochemical studies
| Antibody | Clone | Host | Source | Dilution |
|---|---|---|---|---|
| Anaplastic lymphoma kinase (ALK1) |
ALK01 | Monoclonal mouse | Ventana (Tucson, AZ) | Predilute |
| βF1 T-cell receptor (βF1) | 8A3 | Monoclonal mouse | Thermo Fisher Scientific (Rockford, IL) |
1:25 |
| CD2 | AB75 | Monoclonal mouse | Novocastra (Leica Microsystems, Bannockburn, IL) |
1:100 |
| CD3 | n/a | Polyclonal rabbit | Dako (Carpinteria, CA) | 1:100 |
| CD4 | SP35 | Monoclonal mouse | Dako | 1:200 |
| CD5 | SP19 | Rabbit monoclonal | Ventana | Predilute |
| CD7 | LP15 | Monoclonal mouse | Vector Laboratories (Burlingame, CA) |
1:50 |
| CD8 | C8/144B | Monoclonal mouse | Dako | 1:40 |
| CD20/L26 | L26 | Monoclonal mouse | Ventana | Predilute |
| CD25/interleukin 2 | 4C9 | Monoclonal mouse | Novocastra | 1:400 |
| CD30 | Ber-H2 | Monoclonal mouse | Ventana | Predilute |
| CD56 | 123C3.D5 | Monoclonal mouse | Cell Marque (Rocklin, CA) | Predilute |
| FOXP3 | FOXP3 | Monoclonal mouse | Abcam Inc. (Cambridge, MA) | 1:50 |
| Granzyme B | GrB-7 | Monoclonal mouse | Dako | 1:25 |
| P53 tumor suppressor protein (p53) |
DO-7 | Monoclonal mouse | Dako | 1:100 |
| CD279 | NAT | Monoclonal mouse | Abcam | 1:100 |
| T-cell intracellular antigen-1 (TIA-1) | 2G9A10F5 | Monoclonal mouse | Beckman Coulter (Brea, CA) | 1:400 |