Figure 5. Downstream cascade was further explored and it was shown that hypobaric hypoxia reduces GSK3ß phosphorylation (A) and hence its inactivation whereas enriched environment significantly ameliorate this effect.

In presence of PI3K inhibitor (Wortmannin) enriched environment have little effect on GSK3ß phosphorylation. Inhibition of ERK pathway with U0216 showed no significant effect on GSK3ß phosphorylation. Moreover hypobaric hypoxia also decreases the CREB phosphorylation (B) whereas exposing them with enriched environment normalizes this decreased level of CREB phosphorylation. Similarly inhibition of GSK3ß with AR-A014418 elevates the CREB phosphorylation equivalent to group exposed to enriched environment during hypobaric hypoxia. Data represents Mean ± SEM. In “*” and “#” represents p<0.001 when compared to control and hypoxic group while “?”represents p<0.05 when compared to control group.