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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Traffic. 2013 Mar 20;14(6):650–662. doi: 10.1111/tra.12064

Figure 2. Lysophosphatidylcholine inhibits the Vam7p bypass of anti-Sec17p IgG blocked fusion reactions.

Figure 2

Fusion reactions were treated with buffer or blocked with anti-Sec17p antibody. Reactions were further treated with 150 μM CPZ or 0.033% LPC for 5 minutes preceding the addition of recombinant Vam7p or Vam7pQ283R. In parallel, anti-Sec17p antibody blocked vacuoles were treated with CPZ for 5 minutes followed by LPC for 5 minutes or vice versa before the addition of 400 nM Vam7p or Vam7pQ283R. Error bars represent SEM (n=3)